Figure 1

STEAP2 Knockdown results in a significant reduction in cell proliferation in PC3 cells. (A) qRT-PCR analysis for STEAP2 gene expression in control (PC3^WT and PC3^Sc) and STEAP2-siRNA (PC3^KD). (B) Western blot analysis for STEAP2 protein expression in control (PC3^WT and PC3^Sc) and STEAP2-siRNA (PC3^KD). (Loading control = Β-actin, Black lines represent where the western blot image has been edited for clarity). (C) Growth rate of the cells recorded in cell index (CI) values. PC3 cells monitored over a 72 h period and PC3^KD cells proliferate at a slower rate. (D) The maximum cell index values (i.e. final cell numbers) were significantly reduced in the PC3^KD cells compared to PC3^Sc control (***P < 0.001). (E) qRT-PCR analysis for STEAP2 gene expression in control (LNCaP^WT and LNCaP^Sc) and STEAP2-siRNA (LNCaP^KD). (F) Western blot analysis for STEAP2 protein expression in control (LNCaP^WT and LNCaP^Sc) and STEAP2-siRNA (LNCaP^KD). (Loading control = Β-actin, Black lines represent where the western blot image has been edited for clarity.) (G) LNCaP cells were monitored over a 96 h period and there was no difference observed between transfections. (H) The maximum cell index values were not altered in the LNCaP^KD cells compared to LNCaP^Sc control.