Table 1 Quantitative Real Time PCR Analysis of Array Results.

From: STEAP2 Knockdown Reduces the Invasive Potential of Prostate Cancer Cells

 

Gene

Relative Change in Expression (according to gene array)

qRT-PCR data for WT cells

qRT-PCR data for KD cells

PC3

FGFR4

2.65

2.518*

3.283*

IL1β

−3.64

0.917

0.564*

KiSS1

2.79

1.699*

3.198*

CD82

2.74

1.647*

2.695*

MMP3

2.16

0.864

5.369*

MMP10

4.47

2.739*

4.947*

MMP13

4.19

4.781*

5.587*

SERPINE1

2.70

1.421*

3.191*

LNCaP

CD82

−2.21

0.756

0.478*

IGF1

−2.60

1.174

0.993

MMP7

−4.82

0.881

0.434

MET

4.79

3.523*

4.471

  1. qRT-PCR was carried out on genes detected as altered in the tumour metastasis gene array and analysed in triplicate. Relative expression is depicted normalised to scrambled siRNA treated cells. Significant alterations *p < 0.05.
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