Table 1 Polyacrylamide stock solution compositions.
Gel | %AAm | %Bis | E (kPa)1 | Ratio | 40% AAm (mL) | 2% Bis | UPW (mL) | Total vol. (mL) |
---|---|---|---|---|---|---|---|---|
1 | 5 | 0.02 | 2.0 | 1/250 | 0.625 | 50 µL | 4.325 | 5 |
2 | 8 | 0.15 | 21.5 | 3/160 | 1 | 375 µL | 3.625 | 5 |
3 | 20 | 0.375 | 115.0 | 3/160 | 2.5 | 937.5 µL | 1.563 | 5 |
4 | 20 | 1.5 | 231.8 | 3/40 | 2.5 | 75 mg | 2.5 | 5 |