Figure 1

IraZolve-Mito detects mitochondria in live cardiac and skeletal muscle samples. Representative confocal micrographs showing mitochondria detected with IraZolve-Mito in cardiac (a; enlarged in a^/) and skeletal (c; enlarged in c^/) muscle samples. Endogenous NAD(P)H detected by two-photon microscopy in cardiac (b; enlarged in b^/) and skeletal (d; enlarged in d^/) muscle samples (excited at 740 nm and collected at 474–504 nm). Micrographs showing co-staining of mitochondria with IraZolve-Mito (green in e–e^// and f–f^//) and MitoTracker Red CMXRos (red in e,e^/,e^/// and f,f^/,f^///) in cardiac (e; enlarged in e^/–e^///) and skeletal (f; enlarged in f^/–f^///) muscle samples. Scale bars: 20 µm (a–f) and 5 µm (a^/–d^/, e^/–e^/// and f^/–f^///).