Figure 3
From: Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex

Inhibition of mitochondrial membrane potential in H9c2 rat cardiomyoblasts does not disrupt IraZolve-Mito staining. (a–a//) Micrographs showing co-staining of IraZolve-Mito (green in a; greyscale in a/) with MitoTracker Red CMXRos (red in a; greyscale in a//) in cardiomyoblasts. Scale bars: 20 µm. Cardiomyoblasts were stained with IraZolve-Mito (b,c), MitoTracker Red CMXRos (d,e) or by anti-Cytochrome C antibody (f,g) either under normal conditions (control; b,d,f) or following treatment with FCCP (c,e,g). Scale bars: 20 µm.