Figure 3
TAZ activation increases fat storage and inhibits lipolysis of the mammary gland adipocytes. (A) H&E staining of mammary gland for 8-week WT or MMTV-rtTA/TRE-TAZ mice. (Scale bar = 100 µm). (B) Quantification of the number and size of mammary gland adipocytes in 8-week WT or MMTV-rtTA/TRE-TAZ mice. n = 3 mice per group; 5 fields were counted per group; error bars represent SD; ***p < 0.001 by two-tailed student’s t-test. (C) Measurement of the circulation of triglyceride. n = 3 mice per group; NA, no significant difference by two-tailed student’s t-test. (D) Measurement of ex vivo mammary gland lipolysis. n = 3 mice per group; error bars represent SD; ***p < 0.001 by two-tailed student’s t-test. (E) Real-time qPCR measured expression levels of Atgl, Hsl and Mgl of 8-week WT or MMTV-rtTA/TRE-TAZ mammary gland. n = 3 mice per group; NA, no significant difference by two-tailed student’s t-test. (F) Immunoblot of ATGL and HSL expression in WT or MMTV-rtTA/TRE-TAZ mammary gland. n = 3 WT mice 3 mammary glands; n = 3 MMTV-rtTA/TRE-TAZ mice 6 mammary glands. GAPDH was used as loading control.
