Figure 7
Akt inhibitor attenuated TGF-β1-induced IL-6 production and α-SMA protein expression, and reduced collagen mRNA expression in either the absence or presence of TGF-β1. (a) Akt inhibitor (LY294002) decreased TGF-β1-induced IL-6 production in HCFs (n = 4–8, ***p < 0.001). (b) Akt inhibitor (LY294002) decreased TGF-β1-induced α-SMA protein expression in HCFs (n = 4, *p < 0.05, **p < 0.01, ***p < 0.001). (c) Akt inhibitor (LY294002) decreased collagen mRNA in HCFs in either the absence or presence of TGF-β1 (n = 4, ***p < 0.001). The original immunoblot images are shown in Supplemental Fig. 9.
