Figure 1
From: Automated sorting of neuronal trees in fluorescent images of neuronal networks using NeuroTreeTracer
Tree initialization. (a) Idealized model of a binary segmented soma with 3 emanating neurites. (b) Soma boundary is dilated three times with increasing dilation factor and (c) the symmetric difference of the successive masks is intersected with the image producing the black regions shown in the panel. (d) By computing the centroids of the 6 black regions from panel (c) and then connecting the centroids located on the same branch with the soma, we find the starting location of each neurite and its initial orientation, as indicated by the black arrow.
