Figure 3

Genomic features and stage-specific expression of lncRNAs. (A) Hierarchical cluster analysis of novel long non-coding RNAs. WPGMA is used as the agglomeration method. AU (Approximately Unbiased) in red color represents p-value and BP (Bootstrap Probability) value in green color. AU p-value is computed by multiscale bootstrap resampling method. We combined all samples in the same stage for analysis. (B) Heatmap of stage-specific expression of the novel lncRNAs, which was calculated by the Jensen-Shannon distance. (C) The cumulative curve of the average phastCons score of the novel lncRNAs (blue), mRNA (red) and the genomic background (green). Genomic background was randomly selected from the whole genome. (D) and (E) Length and exon number distribution between porcine lncRNAs and protein coding genes; the blue portion represents the lncRNAs, and the pink portion represents the protein coding genes.