Figure 3 | Scientific Reports

Figure 3

From: JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway

Figure 3

Effect of JIB-04 on the tumor-initiating ability and growth/relapse of tumorspheres. (A) Phase contrast image of tumorspheres derived from cells cultured with DMSO (control), 2 µM JIB-04, or 10 µM salinomycin for 24 h. (B) Percentages of sphere-initiating cells measured by CCK assay. The number of DMSO-treated cells was set as 100 (n = 3). (C) Phase contrast image of primary tumorspheres after treatment with DMSO (control), 2 µM JIB-04, 10 µM JIB-04, or 10 µM salinomycin for 2 days. (D) Viability of primary tumorspheres measured by CCK assay. The number of DMSO-treated cells was set as 100 (n = 3). (E) Phase contrast image of secondary tumorspheres developed from primary tumorspheres without drug treatment for 12 days. (F) Viability of secondary tumorspheres measured by CCK assay. *p < 0.05, **p < 0.01, ***p < 0.001, compared with DMSO.

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