Figure 5 | Scientific Reports

Figure 5

From: JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway

Figure 5

JIB-04 inhibited cell invasion and migration in colorectal CSCs. (A) Cell migration was investigated by transwell assay after treatment with DMSO, 2 µM JIB-04, or 10 µM salinomycin for 24 h (n = 3). (B) Invasive ability was examined in transwells coated with Matrigel after treatment with DMSO, 2 µM JIB-04, or 10 µM salinomycin for 24 h (n = 3). (C) mRNA expression of E-cadherin, vimentin, and N-cadherin after treatment with DMSO, 2 µM JIB-04, or 10 µM salinomycin for 24 h. The mRNA levels of the target genes were normalized to that of GAPDH. The expression level of DMSO-treated cells was set as 1 (n = 3). (D) Protein expression of E-cadherin, EpCAM, and vimentin after treatment with DMSO, 2 µM JIB-04, or 10 µM salinomycin for 24 h. GAPDH was used as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001, compared with DMSO.

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