Figure 2
From: The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation
Expression of cell type specific genes in spaA- cells. Wild-type Ax2 and spaA- cells were plated for development to fruiting bodies (24 h), and total RNA was isolated at 2 h intervals, starting at mounds (12 h). mRNA levels for the prespore genes cotC and pspA, the prestalk gene ecmA, the spore gene SpiA, the constitutively expressed gene Ig7 (A) the transcription factors srfA and bzpF and their respective targets sigF and DDB_G0290419 (B) were measured by qRT-PCR. Data are expressed as fraction of the highest expression obtained with Ax2 for each experiment. Because the timing of peak expression differs between experiments, data from three experiments are plotted separately. Black and grey lines connect the mean values of the three experiments for Ax2 and spaA-, respectively.
