Figure 4
From: The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation
Chromatin immuno-precipitation with SpaA-YFP. SpaA- and spaA-/SpaA-YFP early culminants were dissociated and incubated for 1 h with or without 10 mM 8Br-cAMP. After crosslinking to protein, chromatin was sheared and immuno-precipitated with or without αGFP antibody. The presence of cotC, srfA, spiA and sigF promoter sequences in the immuno-precipitates was determined by qPCR using promoter specific primers (Supplementary Table S2), or cotC coding sequence (CDS) specific primers as control. Amounts of amplified products are normalized to amounts obtained from diluted total cell lysate and expressed as fraction of DNA amplified from promoter regions at 0 h in antibody treated SpaA-YFP expressing cells. Means and SD of 3 experiments. Significant changes (P < 0.05) between some treatments are indicated by asterisks.
