Figure 1
Differential expression of the two hlyCABD operons present in the J96 strain. (a) Percentage of identity at the level of the nucleotide sequence between the operon hly I and hly II of J96 and 536. n.d.: not determined. (b) Hemolytic phenotype of J96, JFV16 (J96ΔII) and JFV21 (J96ΔI) strains on Columbia Blood agar plates. (c) Coomassie blue stained SDS-PAGE (10%) of secreted protein extracts from cultures grown in LB0 at 37 °C up to late-log phase (OD600 nm of 1.0) of the indicated strains. Lane M: molecular mass markers (size in kDa indicated along the left side). The band corresponding to α-hemolysin (HlyA) is indicated with an arrowhead. Full-length gel image is shown in Fig. S6.
