Figure 5
Rapid response of hly II transcriptional expression to zinc addition. (a) Growth curves of the strain JFV3 and EV46 (zur::CmR) in the indicated media by measuring OD600 nm from two independent cultures; mean values are plotted. (b) Transcriptional expression of hly II in cultures of JFV3 and EV46 after zinc addition. Cultures were grown in M-LB up to an OD600 nm of 0.3 when media was either replenished or not with 10 μM ZnCl2. Culture samples were taken right before ZnCl2 addition (0 minutes), and after 15, 30 and 60 minutes. As a control, JFV3 was cultured in M-LB replenished with 10 μM ZnCl2 from the beginning. β-galactosidase activity (expressed in Miller units) was determined from three independent cultures; mean values with standard deviation are plotted.
