Figure 5
Anti-tumor effect of RUNX1 inhibition is SOS1-dependent. (a) Immunoblotting of RUNX1, SOS1 and GAPDH in non-depleted and RUNX1-depleted (RUNX1 K/D) NUGC4 and MKN45 cells transduced with or without lentivirus expressing SOS1 (SOS1 O/E). Cells were treated with 3 μM doxycycline for 48 hours, then lysed for immunoblotting. (b) Restoring SOS1 expression in RUNX1-depleted NUGC4 and MKN45 cells reverts RUNX1-depletion-mediated growth inhibition. The indicated cells were cultured in the presence of 3 μM doxycycline (n = 5). Data are mean ± SEM values. **P < 0.01, by two-tailed Student’s t-test.
