Figure 7
Chb-M’-mediated anti-tumor effect in gastric cancer cells. (a,b) Immunohistochemistry images of SOS1 expression in the MKN45-derived tumor treated with Chb-M’ or control DMSO. NOG mice were subcutaneously-transplanted with MKN45 cell at Day 1. Treatments either by Chb-M’ (320 μg/kg body weight, twice a week, i.v.) or equivalent amount of DMSO was continued from Day 8 until Day 21, then the mice were properly-sacrificed for tumor resection. Resected tumors were fixed with 10% formaldehyde for immunohistochemistry assay. Scale bars 200 μm for low magnification and 25 μm for high magnification (a). SOS1 staining in the MKN45-derived tumor treated with DMSO or Chb-M’ was quantified by Image J (b). (c) Dose-response curves of Chb, Chb-S and Chb-M’ in NUGC4 and MKN45 cells. Cells were treated with the indicated concentrations of PI polyamides or Chb. Forty-eight hours after treatment, cell viability was examined by WST assay (n = 5). (d) Dose-response curves of lapatinib and Chb-M’ in NUGC4 and MKN45 cells. Cells were treated as in (c). Forty-eight hours after treatment, cell viability was examined by WST assay (n = 5). Data are mean ± SEM values. *P < 0.05, **P < 0.01, ***P < 0.001, n.s.; not significant, by two-tailed Student’s t-test.
