Figure 8
SOS1-overexpression confers resistance to the Chb-M’ treatment in gastric cancer cells. (a) Immunoblotting of SOS1 and GAPDH in NUGC4 and MKN45 cells transduced with control or SOS1-expressing lentivirus. Cells were treated with 3 μM doxycycline for 48 hours to induce SOS1 expression, then lysed for immunoblotting. (b) Dose-response curves of Chb-M’ in NUGC4 and MKN45 cells transduced either with the lentivirus expressing SOS1 (SOS1 O/E) or control. Cells were simultaneously treated with 3 μM doxycycline and various concentrations of Chb-M’. Forty-eight hours after treatment, cell viability was examined by WST assay (n = 5). (c) IC50 values of Chb-M’ in NUGC4 and MKN45 cells transduced either with the lentivirus expressing SOS1 (SOS1 O/E) or control. Cells were treated as in (b) (n = 5). Data are mean ± SEM values. *P < 0.05, **P < 0.01, ***P < 0.001, by two-tailed Student’s t-test.
