Figure 1

Schematic of the model building process illustrating sources of data and model outputs. The area indicated by label (1) shows the combination of RECON1 human genome scale metabolic reconstruction and transcriptomic data to form iEC2812. This is then combined, shown in the area labelled (2), with further transcriptomic data to form iHUVEC2552, iHPAEC2588 and iHMVEC2565. These models are compared to produce part of the results of this paper. More transcriptomic data is combined with iHUVEC2552 and metabolic data from cultured cells to produce models of cultured HUVECs treated with LPS and IFNγ to form four models shown in the section labelled (3), both the metabolic data used and the comparison of these four models form part of the results of this paper. The section labelled (4) shows the model of HUEVCs treated with both LPS and IFNγ being combined with metabolic data from the literature to model the endothelial metabolic differences between sepsis survivors and non-survivors, the comparison of these models forms the final part of the results section in this paper.