Table 1 The differential proteins identified by MALDI-TOF/MS.

From: Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm

 

Identified Protein

SYMBLE

SWISSPROT ID

Mr(Da)

Score

Up or Down in low fertility sperm

1

Outer dense fiber protein 2

ODF2

Q5BJF6

73292.6

472

Down

2

A-kinase anchor protein 4

AKAP4

Q5JQC9

93385.1

385

Down

3

Pyruvate kinase

PKM

P14618

65888.8

245

Down

4

T-complex protein 1 subunit gamma

CCT3

P49368

60424.3

80

UP

5

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific

GAPDHS

O14556

44472.8

178

Down

7

ATP synthase subunit beta, mitochondrial

ATP5B

P06576

56524.6

590

Down

8

ATP synthase subunit alpha, mitochondrial

ATP5A1

P25705

59713.6

227

Down

9

Alpha-enolase

ENO1

P06733

47139.3

326

Down

10

Elongation factor 1-gamma

EEF1G

P26641

56114.4

235

UP

11

Fructose-bisphosphate aldolase A

ALDOA

P04075

39395.3

140

Down

12

Tubulin alpha-3C/D chain

TUBA3C

Q13748

49927.6

177

Down

13

Tubulin alpha-3EChain

TUBA3E

Q6PEY2

49884.6

177

Down

14

L-lactate dehydrogenase A-like 6B

LDHAL6B

Q9BYZ2

41916.2

89

Down

15

SRA stem-loop-interacting RNA-binding protein,mitochondrial

SLIRP

Q9GZT3

12341.4

59

UP

16

Mirror-image polydactyly gene 1 protein

MIPOL1

Q8TD10

30255.6

43

UP

17

Uncharacterized protein C22orf31

C22orf31

O95567

32634.3

40

UP

18

E3 ubiquitin-protein ligase RNF34

RNF34

Q969K3

41613.5

46

UP

19

ADP-ribosylation factor-related protein 1

ARFRP1

Q13795

22599.4

34

UP

20

Tubulin beta-6 chain

TUBB6

Q9BUF5

50058.1

76

Down

21

Peroxiredoxin-6

PRDX6

P30041

25019.2

113

Up

22

Sperm protein associated with the nucleus on the X chromosome B1

SPANXB1

Q9NS25

11832.8

127

Down

23

Sperm protein associated with the nucleus on the X chromosome A1/A2

SPANXA1

Q9NS26

11790.7

104

Down

24

Sperm protein associated with the nucleus on the X chromosome C

SPANXC

Q9NY87

10995.4

107

Down

25

Sperm protein associated with the nucleus on the X chromosome D

SPANXD

Q9BXN6

11022.4

360

Down

26

Gamma-enolase

ENO2

P09104

47239

171

Down