Figure 2
AKAP-12 inhibition modifies mitochondrial function and oxidant status in adult human cardiac fibroblasts. AKAP-12 protein (A) expression was measured in AKAP-12-knock-down human cardiac fibroblast. Total mtDNA expression was quantified by RT-PCR (B). Effects of AKAP-12 knock-down on oxidative markers (C,D). Effects of AKAP-12 knock-down on PGC-1α and PHB protein expressions (E). Histogram bars represent the mean ± SEM of 6 assays. For Western blot experiments, the blots were cropped, protein densitometry was expressed in arbitrary units (AU) once normalized to β-actin. For RT-PCR experiments, data was normalized to HPRT and β-actin for cDNA. *p < 0.05 vs Scramble. AKAP-12, A-kinase anchoring protein 12; Total mtDNA, Total mitochondrial DNA; TAC, Total antioxidant capacity; CML, carboxy-methyl-lysine; MDA, malondialdehyde; PGC-1α, peroxisome proliferator-activated receptor-gamma coactivator 1 alpha; PHB, prohibitin.
