Figure 1
From: Neural and behavioural correlates of repeated social defeat
A resident-intruder paradigm was used to induce repeated social defeat in Astatotilapia burtoni males. (a) A 37.85 L tank was divided in two compartments by an opaque blue barrier, and views of neighbouring tanks were obstructed. One fish was allowed to acclimate to each territory for 2 days. (b) On the morning of the trial, the blue barrier was removed between the two compartments revealing a clear barrier with a small escape hole. The intruder was quickly netted from his home territory and placed into the resident’s territory and allowed to interact until he either escaped through the hole or 1 hour had passed. (c) On subsequent trials (days 2–5), the intruder was quickly netted from his territory and placed into the resident’s compartment, where he quickly faded his stereotypical “dominant” coloration. These trials continued until either the intruder escaped or 30 minutes had passed. At the conclusion of all trials the intruder was placed back into his home territory and the blue barrier was replaced (as shown in a) until the subsequent trial.
