Table 3 Thermodynamics of interaction between wild type CohScaB5 and wild-type and mutant variants of ScaDocA.
Dockerin | K a M −1 | Δ G o kcal mol −1 | Δ H kcal mol −1 | −TΔSo kcal mol−1 | N |
|---|---|---|---|---|---|
DocScaA WT | 4.02E8 ± 1.69E8 | −12.14 | −66.66 ± 0.585 | 54.51 | 1.01 |
DocScaA N661A | 2.64E6 ± 4.49E5 | −9.15 | −82.45 ± 2.800 | 73.30 | 0.98 |
DocScaA V662A | 4.78E8 ± 5.03E7 | −12.32 | −68.38 ± 0.110 | 56.05 | 1.02 |
DocScaA V666A | 4.07E8 ± 1.86E8 | −12.20 | −56.56 ± 0.391 | 44.35 | 1.02 |
DocScaA N669A | 3.16E8 ± 6.59E7 | −12.12 | −73.42 ± 0.322 | 61.29 | 1.01 |
DocScaA K670A | 3.67E8 ± 4.33E7 | −12.25 | −75.09 ± 0.184 | 62.83 | 1.02 |
DocScaA V721A | 5.10E8 ± 1.54E8 | −12.35 | −48.70 ± 0.199 | 36.34 | 0.95 |
DocScaA H722A | 2.73E8 ± 7.38E7 | −12.07 | −72.44 ± 0.443 | 60.36 | 1.00 |
DocScaA N661 + N669 | Nb | Nb | Nb | Nb | Nb |
DocScaA V662 + V666 | 1.98E7 ± 1.44E6 | −10.19 | −67.48 ± 0.281 | 57.29 | 0.98 |
DocScaA V662 + V721 | 6.24E7 ± 9.36E6 | −11.05 | −64.33 ± 0.455 | 53.28 | 1.08 |
DocScaA V666 + V721 | 2.25E7 ± 1.58E6 | −10.33 | −56.53 ± 0.238 | 46.20 | 1.01 |
DocScaA V662 + V666 + V721 | 2.91E5 ± 3.79E4 | −7.83 | −64.81 ± 3.59 | 56.98 | 1.04 |
