Figure 1
From: A proteomic analysis of an in vitro knock-out of miR-200c
miR-200c genetic TALEN target sequences and knock-out confirmation. (a) The miR-200c gene is located at chromosome 12p13. TALENs were designed to target the miR-200c 3p drosha processing site. (b) Genomic DNA was extracted from MCtrl and M1, M2 and M3 clones, afterwards the miR-200c gene was amplified by PCR to perform sequencing of the miR-200c loci; MCtrl shows a heterozygous mutation while M1, M2 and M3 show various indels in the proximity of the miR-200c 3p drosha processing site on both alleles. (c) MCF7, MCtrl, M1, M2 and M3 miR-200c expression levels were analyzed by quantitative RT-PCR. Expression of miRNAs is shown as mean of three independent experiments ± SD. ns: no statistical difference, p > 0.05, one-way ANOVA post hoc Bonferroni.
