Figure 2

Contribution of GreA to the growth of F. novicida. (A) Deletion of greA gene in F. novicida strain U112 was confirmed with PCR using primer pair Pr0023/Pr0024. The images were acquired by the gel imaging system (LIUYI). Sizes of molecular markers are indicated at the left in kbp. (B) Loss of GreA protein in F. novicida was detected with western blotting using a mouse polyclonal antibody raised against recombinant GreA protein. The image was acquired by the automatic chemiluminescence image analysis system (Tanon). Size of the protein is indicated at the left in kDa. (C) Growth kinetics of wild-type strain U112 and its derivatives in TSB. The values represent the means ± SD (n = 3) from one of the three independent experiments. (D) Growth of wild-type strain U112 and its derivatives on TSA plates. The image was acquired with the digital camera (Canon, Janpan). (E) Transcription of greA locus genes in wild-type strain U112 and the ΔgreA mutant. Data are the relative transcription levels of each target gene normalized to that of the 16S rRNA gene. Results are shown as the mean fold changes relative to the wild-type U112 strain ± SD (n = 3) from one of the three independent experiments. Statistical significance was determined with unpaired Student’s t test.