Figure 2

Osteosarcoma-derived Sema3A reduces motility, invasion and growth of KHOS cells. In vitro 2D directed (A) and random (B) cell migration of human KHOS cells overexpressing Sema3A (Sema3A^OE) or mock control after 4 and 8 hours as assessed by wound healing and individual cell tracking, respectively. Representative photomicrographs from the 2D directed migration experiment described are shown in panel A, right. (C) In vitro cell invasion of human KHOS cells overexpressing Sema3A (Sema3A^OE) or mock control after 72 hours as assessed by Transwell Chamber assay. Representative photomicrographs from the experiment described are shown in the right. (D) In vitro cell growth of human KHOS cells overexpressing Sema3A (Sema3A^OE) or mock control in the absence of fetal calf serum after 48 hours as assessed by AlamarBlue assay. Representative photomicrographs from the experiment described are shown in the right. A line represents cell front at 0 hours, dotted line represents cell front at 4 hours. Data are mean ± SEM, n = 3 *p < 0.05, **p < 0.01 ***p < 0.001.