Figure 3
From: Bidirectional regulation of bone formation by exogenous and osteosarcoma-derived Sema3A
Osteosarcoma-derived Sema3A inhibits ectopic bone formation in vivo. (A) Graphic representation of para-tibial injection of human KHOS cells overexpressing Sema3A (Sema3AOE) or control (mock) (n = 10, 16 days) in 5 week old female Rj: NMRI nude mice. (B) Bone volume (BV/TV, %) in the tumour-bearing tibia (dashed circle) from the experiments described in panel A. Representative photomicrographs of microCT scan of trabecular bone from the experiment described are shown on the right. Ectopic bone volume (BV %) in the tumour-bearing tibia (C) and fibula (D) from the experiments described in panels A-B. Representative photomicrographs of microCT scans of fibular ectopic bone from the experiment described are shown on the right. Data are mean ± SEM, n = 10 *p < 0.05, **p < 0.01.
