Figure 2
Gene expression of conduction system markers in E11.5 cultures treated with or without ANP and A71915. RT-qPCR analysis was used to monitor HCN4 (A) and Cx40 (B) gene expression levels in cells treated with ANP (1 μg/ml) and or NPRA inhibitor, A71915 (1 µM) for 48 hours. Expression levels were normalized to GAPDH via ∆∆CT method. Relative expression levels were presented as fold changes in comparison to the levels in untreated cultures (Cont). N = 6 experiments per group, each bar represents mean ± SEM, *P < 0.05 Vs. Cont, #P < 0.005 Vs. ANP (1 μg/ml) for both panels, One-way ANOVA with Tukey’s multiple comparisons post hoc test.
