Figure 1

Egr2-mediated PD-L1 induction in CD4⁺ T cells. (a) LAG3 expression in splenocytes from C57BL/6 (B6) mice was analyzed by flow cytometry (FCM). CD25⁺ Tregs: CD4⁺CD25⁺ T cells; naïve T: CD4⁺CD25⁻CD45RB^high T cells; LAG3⁺ Tregs: CD4⁺CD25⁻CD45RB^lowLAG3⁺ T cells. (b) PD-L1 expression in T cell subsets. Histograms are gated on CD4⁺ T cells (left). Data are representative of three independent experiments. Mean fluorescence intensity (MFI) of the indicated T cell subsets is shown (right). (n = 3 per group). *p < 0.001 (Bonferroni’s multiple comparison test) (c) Quantitative PCR assessing Egr2 mRNA expression in the indicated T cell subsets (n = 3 per group). (d) Retroviral constructs of pMIG vector for the transduction of Egr2. (e) Evaluation of PD-L1 expression in Egr2 gene-transduced CD4⁺ T cells. MFI ratio represents the PD-L1 MFI signals of GFP negative versus GFP positive. Plots and histogram are the representative of 3 independent experiments.