Figure 4
From: Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia
miRNA levels in cervical cancer tissues (a). In each surgical specimen, the value of miRNA expression level was corrected for the median value of mucus derived from the normal cervix. The corrected value of the expression level is depicted on the y axis. The available frozen tissues were derived from the enrolled patients in the real-time RT-PCR cohort (tissue ID in Supplementary Table S1). The green and orange bars indicate the tissues from SCC and AD, respectively. SCC: squamous cell carcinoma, AD: adenocarcinoma. Both cervical cancer cells and endothelia in the stroma were stained with a miR-126 probe by in situ hybridization. (b). In situ hybridization with a specific has-mir-126 locked nucleic acid probe was performed on the formalin-fixed paraffin-embedded surgical specimens. miR-126 was highly expressed in the endothelia as well as the cytoplasm of cervical cancer cells. Adenocarcinoma (T041 in Fig. 4a) is shown in the left photo (×4) and the right photo (×10). Normal glands are stained whereas the cytoplasm of cancer cells is stained in T041(×4). Endothelia in the surrounding stroma and cancer cells are stained in T041(×10). Squamous cell carcinoma is shown inT043 (×4) and (×10). The cytoplasm of cancer cells and endothelia in the surrounding stroma are stained.
