Table 2 BNA primer, padlock probe and detection probe nucleotide sequences.

Transcript variant	Exon targets (probe)	Nucleotide sequences (5′→3′)
Transcript variant	Exon targets (probe)	BNA primer	Padlock probe	Detection probe
METexon7-8^wt	Exon 7-8 (MET^7,8)	CAGCCATAGGACC GTATTTCGGCGA	GATCCTGTAATAACAAGTATGTGACATACTGACAAAGTAGCCGTG *ACTATCGACT*TGACCAGTTAGCAAAACAGTACATTCTCCTATGTG	Cy5-AGTAGCCGTGACTATCGACT
MET^Δ7-8	Exon 6-9 fusion (MET^6–9)	GGGCTGGGGTATA ACATTCAAGAAT	TGTGTCAAACAGTATTCTTGGTGACATACTGACACCTCAATGCACA TGTTTGGCTCCTGACCAGTTAGCAAGTGAGAGCACGATGAATAC	TexRd-XN-CCTCAATGCACATGTTTGGCTCC
METexon14^wt	Exon 14 (MET¹⁴)	CACTTCGGGCACTT ACAAGCCTATC	CACTCCTCATTTGGATAGGCGTGACATACTGACAAAGTAGCCGTG *ACTATCGACTT*GACCAGTTAGCAAACGCTACGATGCAAGAGTACA	Cy5-AGTAGCCGTGACTATCGACT
MET^Δ14	Exon 13-15 fusion (MET^13–15)	TCGGCATGAACCG TTCTGAGATGAA	ATCAGTTTCCTAATTCATCTGTGACATACTGACACCTCAATGCACAT GTTTGGCTCCTGACCAGTTAGCAAAAGAGAAAGCAAATTAAAG	TexRd-XN-CCTCAATGCACATGTTTGGCTCC
EGFR^wt	Exon 2-3 (EGFR^2,3)	AGGGCAATGAGGA CATAACCAGCCA	ACCATCCAGGAGGTGGCTGGGTGACATACTGACAAAGTAGCCGT *GACTATCGACT*TGACCAGTTAGCAAAATGATCTTTCCTTCTTAAAG	Cy5-AGTAGCCGTGACTATCGACT
EGFRvIII	Exon 1-8 fusion (EGFR^1–8)	CTCGGACGCACGAGCCGTGATCTGT	GTAATTATGTGGTGACAGATGTGACATATGAAACCTCAATGCTGCTGC TGTACTAC TGACCAGTTAGCAGGCTCTGGAGGAAAAGAAAG	TexRd-XN-CCTCAATGCTGCTGCTGTACTAC

BNA primer Bridged Nucleic Acids are depicted in bold. Padlock probe target-specific sequences are depicted in italic. Detection probe complementary sequences in the padlock probe are coloured bold italic (wild-type) or italic underline (mutant).

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