Figure 1 | Scientific Reports

Figure 1

From: Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance

Figure 1

The E198A mutation in the P. xanthii β-tubulin confers resistance to the MBC fungicide carbendazim. (A) The P. xanthii SF60 (A198) isolate grew on zucchini cotyledons treated with increasing concentrations of carbendazim until 3.3 mM. However, the growth of isolate 2086 (E198) was completely abolished at 1.5 mM. (B) Coding sequences of the P. xanthii TUB2 gene were cloned in the yeast expression vector pREP-3X and plasmids were then transformed into S. pombe strain RA4134. The sensitivity of S. pombe transformants to fungicides was evaluated by drop assay onto PMG medium lacking thiamine, without fungicide or amended with the MBC fungicides carbendazim (3.3 mM) or benomyl (0.1 mM). The strain expressing the resistant β-tubulin (pREP-A198), but not the strain expressing the sensitive β-tubulin (pREP-E198), grew in the presence of 3.3 mM carbendazim. Identical results were obtained using the MBC fungicide benomyl (0.1 mM).

Back to article page