Figure 4 | Scientific Reports

Figure 4

From: Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance

Figure 4

Characterization of the interaction between carbendazim and P. xanthii β-tubulins by monitoring the intrinsic tryptophan fluorescence. (A) Fluorescence spectra of the MBC-sensitive and MBC-resistant β-tubulins. Sensitive and resistant β-tubulins were incubated in the absence (■) and presence of different concentrations of carbendazim: 1.3 (△), 2.5 (▲), 3.3 (○), 5.2 (●), 15.0 (□) and 25.0 () mM. (B) A plot against log [carbendazim concentration] according to the modified Stern-Volmer equation for MBC-sensitive (solid line) or MBC-resistant (dashed line) β-tubulin. F 0 and F are the peaks of fluorescence intensities of β-tubulin in the absence and presence, respectively, of the quencher (carbendazim).

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