Figure 6

Emission spectra of the intrinsic fluorescence of Vip3Af1(WT) and mutant Vip3Af proteins with reduction of their insecticidal activity. Proteins (3–8 µg/mL) were excited at 280 nm and the emission was scanned from 300 to 450 nm. “P”: protoxin; “T”: protoxins treated with 20% trypsin (wt/wt) at 37 °C for 1 h. The maximum emission peak is indicated within brackets.