Figure 8
From: Specific features of human monocytes activation by monophosphoryl lipid A
Induction of homo- and cross-tolerance. Human adherent mononuclear cells were pretreated for 20 h with either conventional smooth E. coli O111:B4 LPS (cLPS) (100 ng/ml), highly purified S. abortus equi LPS (hpLPS) (100 ng/ml), highly purified rough S. minnesota R595 LPS (ReLPS) (100 ng/ml) or MPLA (1 µg/ml). After elimination of the supernatants and washings, cells were re-challenge with either cLPS (A), hpLPS (B), Pam3CysSK4 (C) or Pam2CysSK4 (D), and TNF production was assessed 20 h later. The results are expressed as percent of the production of individual donors to the different agonists used for challenge in cells, which had not been pre-treated with TLR agonists. Each dot represents individual donors. *p ≤ 0.5; **p < 0.01; ***p < 0.001; ****p < 0.0001.
