Figure 6

Potentiation of ASIC1a/2a by high concentration PcTx1 in mouse cortical neurons. (a) Traces of pH6.8-induced current from a single wild-type neuron in control buffer and in the presence of 10 nM PcTx1, 100 nM PcTx1, 100 nM PcTx1 + 300 μM Zn and 100 nM PcTx1 + 300 μM Zn + 500 μM amiloride. (b) Traces of pH6.8- or pH4.5-induced current from a single ASIC1^−/− neuron in control buffer (for both pH6.8 and pH4.5) and (for pH6.8 only) in the presence of 100 nM PcTx1, 100 nM PcTx1 + 300 μM Zn and 100 nM PcTx1 + 300 μM Zn + 500 μM amiloride. (c) Summary of pH6.8, pH6.0 or pH4.5-induced currents under conditions indicated for wild-type and ASIC1^−/− neurons. Numbers in the parentheses indicate the number of neurons tested under each indicated condition. The conditioning pH was 7.4 for all the experiments in Fig. 6.