Figure 1 | Scientific Reports

Figure 1

From: A single amino acid substitution in the Bombyx-specific mucin-like membrane protein causes resistance to Bombyx mori densovirus

Figure 1

Mapping of nsd-1 in linkage group 21. (A) SNP markers and linkage analysis. The upper panel indicates the positions of the SNP markers in BAC end sequences21. Distances between the markers are shown in centiMorgans (cM). In the lower panel, the dotted arrows indicate the results of rough mapping of the region linked to nsd-1. (B) Fine mapping on the scaffold sequence. The dotted arrows indicate the results of linkage analysis, which was performed to narrow the nsd-1 region. The nsd-1 locus was located within an area of approximately 400-kb-long between 7,402,527 and 7,799,223 bp on Bm_scaf7. This region contains five predicted genes: BGIBMGA001390, 001596, 001389, 001388, and 001597. (C) RT-PCR analysis of predicted genes located within the nsd-1 region in resistant and susceptible strains. The internal control was 18S ribosomal RNA. Lane 1, C124 (resistant); 2, p50T (resistant); 3, J124 (susceptible); and 4, J150 (susceptible). A mixture of λ/HindIII and Φ×174/Hae III (New England Biolabs) was used as a DNA size marker.

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