Figure 3 | Scientific Reports

Figure 3

From: A single amino acid substitution in the Bombyx-specific mucin-like membrane protein causes resistance to Bombyx mori densovirus

Figure 3

Expression profiles of nsd-1. (A) Tissue-specific expression of nsd-1 in a resistant strain, p50T. CNS, central nervous system; SG, silk gland; FB, fat body; HC, hemocyte; TES/OV, testis and ovary; FG, foregut; AMG, anterior midgut; MMG, middle midgut; PMG, posterior midgut; HG, hindgut; MT, Malpighian tubule. Primer sets were designed to amplify nsd-1 (upper) and ribosomal 18S (lower) as a positive control. (B) Stage-specific expression of nsd-1 in the resistant strain p50T. E, eggs at on days 0, 4, and 8; L, whole larvae at on day 0 (first to fifth instar); P, whole pupae at on days 0 and 5; A, whole adult at on day 0. (C) Immunohistochemical analysis of NSD-1 in the larval anterior midgut. The upper panels show immunofluorescence visualization of NSD-1. The lower panels show control experiments with pre-immune serum. The right panels are zoomed-in images of the dotted boxes in the corresponding left panels. The sections were incubated with anti-NSD-1 antibody (1:200) or pre-immune serum followed by a secondary antibody labeled with AlexaFluor488 (1:200) (green) and counterstained with DAPI (1:1000) (blue). Scale bar: 200 μm.

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