Figure 2 | Scientific Reports

Figure 2

From: Dysregulation of tristetraprolin and human antigen R promotes gastric cancer progressions partly by upregulation of the high-mobility group box 1

Figure 2

HuR was downregulated by TTP, and dysregulation of TTP and HuR regulated the expression of HMGB1 in different ways. (a–c) TTP regulated the expression of HuR and HMGB1 at the mRNA level. (d) HuR upregulated the expression of HMGB1 at the translational level. (e) The HMGB1 mRNA decay curve in actinomycin D-treated MGC-803/TTP and MGC-803/pcDNA cells. The half-life of HMGB1 mRNA in MGC-803/TTP cells was shorter than that in MGC-803/pcDNA cells after 6 hours of actinomycin D treatment. The data were represented as the mean ± SD, r2: goodness of fit, **p < 0.01. (f) Luciferase assay in 293 T cells revealed that AREs within the HMGB1 mRNA 3′UTR mediated the inhibitory of TTP. And the schematic showed the potential binding sites of TTP within the HMGB1 mRNA 3′ UTR. Results shown on the graph represent the mean ± S.D. of three independent experiments, **p < 0.01.

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