Figure 1
From: Caveolin-3 differentially orchestrates cholinergic and serotonergic constriction of murine airways
Immunofluorescence labelling for cav-3 in cav-3+/+ and cav-3−/− mice. Representative images for cav-3+/+ (n = 4 animals) and cav-3−/− mice (n = 5 animals). (a–d) Cav-3 immunoreactivity is seen in the cardiac muscle cells (arrow), tracheal epithelium (arrowhead), tracheal and bronchial SMC (double arrowhead), and cardiomyocytes of the pulmonary vein (dashed arrow) and arterial smooth muscle (double arrow) in cav-3+/+ mice. (d) SMC are shown in the left extrapulmonary bronchus and the tracheal bifurcation is also shown by a dotted line. No cav-3 labelling is noted in cav-3−/− mice. Bar = 50 µm. (e) Specificity control of the anti-cav-3 antibody. No cav-3 immunolabelling is present in cav-3−/− mouse samples, while the antibody recognizes a single 20 kDa protein band in protein extracts from cav-3+/+ mouse samples. Our own laboratory polyclonal antibody to succinate dehydrogenase complex, subunit A (SDH-A) labels a protein band at 70 kDa in all cav-3−/− and cav-3+/+ mouse samples as a reference control.
