Figure 6
From: Caveolin-3 differentially orchestrates cholinergic and serotonergic constriction of murine airways
Changes in constrictor force and reactivity of various extrapulmonary airway segments from cav-3+/+ (WT) and cav-3−/− (KO) mice induced by 5-HT. In the organ bath recordings, each point represents the mean number of animals (n) ± SEM. After equilibration, baseline tension was adjusted to 0.5 g. Baseline was set as 100% and the maximum response at each concentration was calculated. (a,b) Changes in constrictor force after additive application of 5-HT. 5-HT induces concentration-dependent contraction in the cranial and middle part of trachea from cav-3+/+ mice, whereas the trachea of cav-3−/− mice and the extrapulmonary bronchi are not responsive to 5-HT. (c,d) Comparison of constrictor response (force and reactivity) between both mouse strains. Cav-3+/+ mice showed significant constriction in the cranial and middle part of the trachea, whereas there was no significant constriction in the caudal part and the extrapulmonary bronchi. In contrast to this neither the tracheal segments nor the extrapulmonary bronchi constricted in cav3−/− mice. All tracheal segments of cav3+/+ mice showed a significant reactivity, whereas the reactivity of the extrapulmonary bronchi was not significant. Reactivity was abolished in cav-3−/− mice. Data were analyzed with One-Way ANOVA or the Kruskal-Wallis test followed by Dunnett’s test for multiple comparisons, depending on normal distribution established with the Shapiro-Wilk normality test. (e) Relative transcript levels of 5-HT1BR and 5-HT2AR genes standardized on internal β-microglobulin (β-MG) levels. Results for the cav-3−/− tissues are presented relative to the results for cav-3+/+ tissues set to 1 to appreciate its potential differences independent from individual assay performance. Data are presented as mean ± SEM (Student’s t-test, *p < 0.05; n = 4 mice per genotype).
