Figure 7
From: Caveolin-3 differentially orchestrates cholinergic and serotonergic constriction of murine airways
Bronchoconstrictor response to muscarine of peripheral bronchi from cav-3+/+ (WT) and cav-3−/− (KO) mice. Videomorphometric analyses of PCLS, depicted are changes in the luminal area. Data are presented as mean of number of bronchi (n)/number of animals ± SEM. (a) Muscarine-mediated concentration-dependent decreases in the luminal airway area in cav-3+/+ and cav-3−/− mice. KCl (60 mM) was applied as a viability control for 5 min, and the pre stimulus value was set as 100%. In response to different concentrations of muscarine (10 nM–100 µM) a dose dependent continuous contraction was observed in bronchi of cav-3+/+ and cav-3−/− mice. (b) Depicted are the sigmoidal concentration-response curves (plotted using the Hill equation) of concentration versus luminal area reduction of peripheral bronchi of cav-3+/+ and cav-3−/− mice. The Student’s unpaired t-test was used to analyze values for Emax and pEC50. Bronchi of cav-3−/− mice responded with a stronger contraction (p ≤ 0.05) and a difference in pEC50 (p ≤ 0.05). (c) Relative transcript levels of muscarinic acetylcholine receptor subtypes 2 and 3 (M2R and M3R) genes standardized on internal β-microglobulin (β-MG) levels. Results for the cav-3−/− tissues are presented relative to the results for cav-3+/+ tissues set to 1 to appreciate its potential differences independent from individual assay performance. Data are presented as mean ± SEM (Student’s t-test, *p < 0.05; n = 4 mice per genotype). No differences in M2R and M3R expression were observed between cav-3−/− and cav-3+/+ tracheal SM, extrapulmonary bronchi and lung (with intrapulmonary bronchi).
