Figure 2 | Scientific Reports

Figure 2

From: LncRNA OIP5-AS1 is overexpressed in undifferentiated oral tumors and integrated analysis identifies as a downstream effector of stemness-associated transcription factors

Figure 2

Prediction of miRNA targeted by OIP5-AS1 and possible interactions. (a) Nucleotide alignment of mature sequences of mouse mmu-miR-7a-5p (top) and hsa-miR-7–5p (bottom) shows highly conserved. (b) Crustal alignment of human OIP5-AS1 (top) and mouse Oip5-as1 (middle) and the interacting site of miR-7 (human/mouse) (bottom). Green lines represent the miRNA seed sequence interaction with the lncRNA and red star indicating the conserved interaction site in human/mouse lncRNA for the candidate miRNA binding. (c) Position of predicted miRNA binding sites in the OIP5-AS1 transcript (top) and their corresponding hybridization energy (kcal/mol) with the base position of the seeds (bottom). The full-length transcript of OIP5-AS1 (1894 nt) has multiple interaction sites for the miRNAs. (d) Interaction network of predicted miRNAs (nodes in green) and their downstream target mRNAs (nodes in blue). (e) Six-set Venn diagram of the miRNAs selected in this study and number of shared mRNA targets. BCL11A, KMT2A and STYX were commonly targeted by all the 6 miRNAs. miR-30a-5p, miR-30b-5p, miR-338-3p and miR-22-3p shared maximum common downstream targets.

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