Figure 3

Expression profiling of predicted miRNAs and their downstream target genes. (a) Expression levels of predicted miRNAs in oral tumors and normal tissues. Except for miR-22-3p and miR-30b-5p, other miRNAs are significantly downregulated in oral tumors. (b) Expression levels of predicted miRNAs in oral tumors between cell differentiation status. miR-137 which shares the least common downstream target and miR-181a-3p which don’t have interaction site with OIP5-AS1 and nor seed match for stemness TFs was upregulated in undifferentiated oral tumors. (c) Expression of CELF1, KMT2A and KMT2C were significantly overexpressed in oral tumor compared with normal tissues. (d) Expression levels of CELF1, KMT2A and KMT2C in oral tumors that overexpressed OIP5-AS1 compared to OIP5-AS1 low expressed tumors. All the 3 mRNAs were upregulated in OIP5-AS1 overexpressed oral tumors with CELF1 having statistical significance. (e) CELF1, KMT2A and KMT2C were overexpressed in oral tumors with undifferentiated cellular pathology. (f) Undifferentiated oral tumors with high levels of OIP5-AS1 also upregulated CELF1, KMT2A and KMT2C compared with undifferentiated tumors with low OIP5-AS1. CELF1 and KMT2A which has the maximum number of predicted miRNAs binding overexpressed significantly than the KMT2C with the minimum number of predicted miRNAs binding. Statistical significance represented as ** for P < 0.01, *** for P < 0.001 and **** for P < 0.0001 (two-tailed Student’s t-test).