Figure 1
Device illustration and culture conditions. (A) The LumeNEXT method49 was used to create the two-layered microfluidic devices. (A) depicts device construction and use, and (B) shows a detailed device schematic. (C) The platform allows the investigation of stromal cells on duct function and estrogenic response, as the duct can be cultured in a collagen matrix, as well as cultured in a collagen matrix that has stromal cells embedded throughout. An example of a device array is shown in (D), where 24 devices are plasma bonded to a cover slip attached to an omni tray.
