Figure 2

MCF7s form confluent polarized ductal structures. The (A) bottom plane and (B) cross-section of MCF7-derived lumens stained with F-Actin (red) and nuclei (blue) were imaged. (C) To evaluate apical-basal polarity, samples were stained for nuclei (blue), laminin-5 (green; basal), and golgi (red; apical). Scale bars represent 100 μm.