Figure 5
From: Role of IGF-1R in ameliorating apoptosis of GNE deficient cells
Cell cycle analysis of GNE deficient cells: The cell cycle analysis using propidium iodide was done by Flow Cytometry. (A) Representative histogram of the distribution of GNE deficient cells and GNE mutant cells in various phases of the cell cycle. Cells were treated with 5 nM IGF-1 alone, Sialic acid alone or a combination of both (IGF-1 + SA) (B) Graphical representation of cells in sub G0/G1 phase (% apoptosis).The statistical significance was assessed by One-way ANOVA test followed by Bonferroni post tests, P values for *, ** and *** are <0.05, <0.001 and <0.0001, respectively. (C) Graphical representation showing fold change in cell survival which is calculated by taking the ratio of percentage sub G0/G1 population cells (represented by R2 in histograms 5A) obtained from 5B of IGF-1 treated versus untreated, SA treated versus untreated and IGF-1 + SA treated versus untreated, normalized to vector control.
