Figure 3
Knockdown of lincDUSP potentiates apoptosis induction in vitro. (A,B) Caspase 3/7 GLO Assay was performed in V703 (Panel A) and V481 (Panel B) cells treated with 1.0 uM doxorubicin (Dox) for indicated timepoints 24 hours post-transfection with indicated GapmeRs. The knock down of lincDUSP was associated with increased Caspase 3/7 activity in both cell lines. P-value generated using student’s t-test; error bars = SE (n = 3). (C) Flow cytometry analysis of surface Annexin V in V481 cells transfected with control or lincDUSP-specific GapmeRs. At 48 hours post-transfection, cells were treated with 2.0 µM doxorubicin for 4 hours prior to staining. (D) Quantitation of Annexin V flow cytometry data. P-value generated using student’s t-test; error bars = SE (n = 4 from two independent experiments).
