Figure 3 | Scientific Reports

Figure 3

From: A Phase I Clinical Trial with Ex Vivo Expanded Recipient Regulatory T cells in Living Donor Kidney Transplants

Figure 3

In process testing of Tregs suppressive function: Suppression assays were performed. (R) denotes recipient PBMC’s; (Sx) denotes irradiated healthy allogeneic PBMC’s (stimulators); (Rx) denotes irradiated responder cells used as a control to keep cell number consistent across groups. (A) Graph shows raw count per minutes (±SD in triplicate cultures) of thymidine incorporation assay after six days of culture (representative). (B) Average (±SD) of percent suppression (n = 9) at varying Treg: Tresp ratios throughout clinical expansion protocol. Percent suppression calculated as described in materials and methods.

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