Figure 10
Substrate rigidity controls nuclear localization of YAP1 in ovarian cancer cells. (A) SKOV3 cells plated on FN-coated hydrogels with elastic moduli of 3 kPa, 25 kPa, and 125 kPa, or on FN-coated glass coverslips, as indicated, were allowed to adhere for 18 h before being fixed and stained with phalloidin, YAP1 antibody, and DAPI to visualize F-actin, YAP1, and nuclei, respectively. Yellow dotted lines through the cells in the overlay images (last column) were used to generate the intensity linescans shown in the next panel. (B) Plotted intensity of YAP1 and DAPI signals through the linear regions of interest depicted in (A). The y-axes represent relative fluorescence intensity of the indicated signals and the x-axes represent the position along the linear ROI. (C) Quantification of YAP1 localization given as a ratio of YAP1 signal in the nucleus to that in the cytoplasm for cells on the indicated substrates. The graph depicts all measure values (colored symbols) and the mean values (±s.d.; n = 34, 14, 22, and 16 cells for 3 kPa, 25 kPa, 125 kPa, and glass, respectively; ***p = 0.0025; ****p < 0.0001 using Mann-Whitney tests).
